The fuel assembly locking apparatus comprises a rotatable plate capable of simultaneously and positively engaging all four legs of the bottom nozzle of a fuel assembly for uniformly holding the fuel assembly while the spent fuel rods are pulled from the fuel assembly. The rotatable plate has four notched corners arranged to be rotated over a ledge on each leg of the bottom nozzle of the fuel assembly thereby preventing movement of the fuel assembly. The rotatable plate is also capable of being rotated 45.degree. so that the four corners of the plate are rotated clear of the legs of the bottom nozzle of the fuel assembly thereby allowing the fuel assembly to be placed on or removed from the locking apparatus. The locking apparatus is also provided with a torque actuator for remotely rotating the plate.