An improved manually operable needle threading device is provided of the character including a housing having an upwardly extending tubular needle receiving support and a displaceable needle threader member in the housing for displacing a thread through openings in the needle support and the eye of a needle therein. The improvements include a slot extending upwardly through the needle support from one of the openings therein to facilitate removal of the threaded needle when one end of the thread is fixed against movement, and an improved actuating arrangement for displacing the needle threader member relative to the needle support.