发明授权
- 专利标题： Method for determining bacterial endotoxin and kit therefor
- 专利标题（中）： 确定细菌内毒素的方法及其试剂盒
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申请号： US441810申请日： 1982-11-15
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公开(公告)号： US4495294A公开(公告)日： 1985-01-22
- 发明人: Chizuko Nakahara , Shigenori Tanaka , Hiroshi Tamura , Akiyoshi Matsumoto
- 申请人： Chizuko Nakahara , Shigenori Tanaka , Hiroshi Tamura , Akiyoshi Matsumoto
- 申请人地址： JPX Tokyo
- 专利权人： Seikagaku Kogyo Co., Ltd.
- 当前专利权人： Seikagaku Kogyo Co., Ltd.
- 当前专利权人地址： JPX Tokyo
- 优先权： JPX56-182190 19811116
- 主分类号： G01N33/52
- IPC分类号： G01N33/52 ; G01N33/50 ; G01N33/579 ; C12Q1/00 ; C12Q1/36 ; C12Q1/38
摘要：
Bacterial endotoxin in an assay sample is determined by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 at a pH of not more than 3; and a kit therefor, a receptacle containing (a) an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 e.g. perchloric acid, trichloroacetic acid, nitric acid, for treating the assay sample and a receptacle containing (b) an amoebocyte lysate component or horseshoe crab. The acid treatment of the assay sample results in improved accuracy, reliability and reproducibility of the bacterial endotoxin determination.
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