发明授权
- 专利标题: Reducing non-specific background in immunofluorescence techniques
- 专利标题(中): 减少免疫荧光技术中的非特异性背景
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申请号: US539872申请日: 1983-10-07
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公开(公告)号: US4582791A公开(公告)日: 1986-04-15
- 发明人: Pyare L. Khanna , Jimmy D. Allen , Ian Gibbons
- 申请人: Pyare L. Khanna , Jimmy D. Allen , Ian Gibbons
- 申请人地址: CA Palo Alto
- 专利权人: Syntex (U.S.A.) Inc.
- 当前专利权人: Syntex (U.S.A.) Inc.
- 当前专利权人地址: CA Palo Alto
- 主分类号: G01N33/533
- IPC分类号: G01N33/533 ; G01N33/536 ; G01N33/542 ; G01N33/53 ; G01N33/554 ; G01N33/571
摘要:
Novel compositions and methods are provided for detecting the presence of a material of interest in a specimen on a solid surface. A composition useful for detecting the presence of a material of interest in a specimen comprises (1) a detector conjugate comprising a fluorescing moiety bonded to a compound capable of specific binding with the material of interest, or a derivative thereof, and (2) a non-detector conjugate comprising a poly(amino acid) and a compound having substantial structural and charge similarity to the fluorescing compound and no observable fluorescence, or low level fluorescence of a different wavelength than that of the fluoresing compound, in the region of fluorescence of the fluorescing compound. In the method of the invention, a specimen, on a solid surface, is combined with the detector conjugate and the non-detector conjugate, and the combination is incubated. Unbound conjugates are removed from the solid surface by washing and then the surface is irradiated with light having a wave length absorbed by the fluorescing compound. The fluorescent light emitted by the combination is observed and is a function of the amount of the material of interest present in the specimen.
公开/授权文献
- US5695314A Apparatus and method for singling stacked cards 公开/授权日:1997-12-09