发明授权
- 专利标题: Homogeneous enzyme specific binding assay on non-porous surface
- 专利标题(中): 在无孔表面上的均相酶特异性结合测定
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申请号: US561987申请日: 1983-12-14
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公开(公告)号: US4629690A公开(公告)日: 1986-12-16
- 发明人: Litai Weng , Ian Gibbons , Edwin F. Ullman
- 申请人: Litai Weng , Ian Gibbons , Edwin F. Ullman
- 申请人地址: CA Palo Alto
- 专利权人: Syntex (U.S.A.) Inc.
- 当前专利权人: Syntex (U.S.A.) Inc.
- 当前专利权人地址: CA Palo Alto
- 主分类号: G01N33/542
- IPC分类号: G01N33/542 ; G01N33/58 ; G01N33/543
摘要:
Enzyme channeling assay is provided employing a solid non-porous surface to which is bound a member of a specific binding pair and an enzyme. A complementary member of a specific binding pair is conjugated to second enzyme, so that the amount of second enzyme which becomes bound to said solid non-porous surface by the binding of a specific binding pair(s) is related to the amount of analyte in the liquid assay medium with which the solid surface is in contact. The first and second enzymes are related by the product of one being the substrate of the other. The turnover of the substrate formed by one of the enzymes results in a detectable product, which can be related to the amount of analyte in the medium.
公开/授权文献
- US5022662A Gasket with a fluid hole regulation device 公开/授权日:1991-06-11