An analysis material sheet is disclosed which is comprised of layers of material with strong adhesion for one another. The sheet includes a water-impermeable support having provided directly or indirectly thereon a layer of fabric. The fabric contains a hydrophobic organic polymer fiber and is physically activated on at least one surface thereof, with the physically activated surface being adhered directly or indirectly to the support. A reagent layer can be provided directly or indirectly on the liquid sample-spreading layer of fabric. By utilizing a fabric containing a hydrophobic organic polymer fiber, body fluids to be analyzed can be quickly and uniformly spread and by utilizing the physically activated surface of the fabric, strong adhesion between the layers can be obtained.