发明授权
US5024935A Dye-providing composition, diagnostic test kit and their use in method for ligand determination using peroxidase labeled-receptor 失效
染料提供组合物,诊断试剂盒及其在使用过氧化物酶标记受体的配体测定方法中的应用

  • 专利标题: Dye-providing composition, diagnostic test kit and their use in method for ligand determination using peroxidase labeled-receptor
  • 专利标题(中): 染料提供组合物,诊断试剂盒及其在使用过氧化物酶标记受体的配体测定方法中的应用
  • 申请号: US136166
    申请日: 1987-12-18
  • 公开(公告)号: US5024935A
    公开(公告)日: 1991-06-18
  • 发明人: Gregory J. McCluneJohn F. Bishop
  • 申请人: Gregory J. McCluneJohn F. Bishop
  • 申请人地址: NY Rochester
  • 专利权人: Eastman Kodak Company
  • 当前专利权人: Eastman Kodak Company
  • 当前专利权人地址: NY Rochester
  • 主分类号: C12Q1/28
  • IPC分类号: C12Q1/28 G01N33/76
Dye-providing composition, diagnostic test kit and their use in method
for ligand determination using peroxidase labeled-receptor
摘要:
A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydrogen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from about 10,000:1 to about 100:1. The dye-providing composition can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particularly useful for the determination of human chorionic gonadotropin (hCG).
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