发明授权
US5085989A Method and reagent for quantitative analysis of 3-oxo-5.beta.-steroid
失效
3-OXO-5（BETA）-STEROID的定量分析方法和试剂
- 专利标题： Method and reagent for quantitative analysis of 3-oxo-5.beta.-steroid
- 专利标题（中）： 3-OXO-5（BETA）-STEROID的定量分析方法和试剂
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申请号： US574525申请日： 1990-08-28
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公开(公告)号： US5085989A公开(公告)日： 1992-02-04
- 发明人: Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata
- 申请人： Koji Ushizawa , Takae Shigihara , Maki Akemichi , Toshiyuki Akimoto , Senkichi Nagasaki , Miyoshi Hirata
- 申请人地址： JPX Tokyo
- 专利权人： Daiichi Pure Chemicals Co., Ltd.
- 当前专利权人： Daiichi Pure Chemicals Co., Ltd.
- 当前专利权人地址： JPX Tokyo
- 优先权： JPX61-268313 19861111
- 主分类号： C12Q1/32
- IPC分类号： C12Q1/32
摘要：
A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.
公开/授权文献
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