发明授权
- 专利标题： Assay for dioxins
- 专利标题（中）： 测定二恶英
-
申请号： US300725申请日： 1989-01-25
-
公开(公告)号： US5128244A公开(公告)日： 1992-07-07
- 发明人: Alan P. Poland , Christopher A. Bradfield , Edward N. Glover , Andrew S. Kende , Frank H. Ebetino
- 申请人： Alan P. Poland , Christopher A. Bradfield , Edward N. Glover , Andrew S. Kende , Frank H. Ebetino
- 申请人地址： WI Madison
- 专利权人： Wisconsin Alumni Research Foundation
- 当前专利权人： Wisconsin Alumni Research Foundation
- 当前专利权人地址： WI Madison
- 主分类号： G01N33/53
- IPC分类号： G01N33/53
摘要：
A screening assay for recognizing the presence of dioxins (and other related toxins) in a sample is disclosed. In one aspect of the invention, Ah receptor from mice and a radioactively labelled halogenated dioxin are used in a competitive binding assay to test for the presence of toxins. The label is .sup.125 I substituted directly on the main dioxin structure. The relative binding of the toxin in the samples (in competition with labelled dioxin) for Ah receptor can be compared against standard curves. A kit is provided for running such an assay and a preferred .sup.125 I ligand is provided.
公开/授权文献
信息查询
IPC分类:
