发明授权
- 专利标题: In vitro propagation of embryonic stem cells
- 专利标题(中): 胚胎干细胞的体外繁殖
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申请号: US477960申请日: 1990-05-31
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公开(公告)号: US5166065A公开(公告)日: 1992-11-24
- 发明人: Robert L. Williams , Nicholas M. Gough , Douglas J. Hilton
- 申请人: Robert L. Williams , Nicholas M. Gough , Douglas J. Hilton
- 申请人地址: AUX Kew
- 专利权人: Amrad Corporation Limited
- 当前专利权人: Amrad Corporation Limited
- 当前专利权人地址: AUX Kew
- 优先权: AUX9644/88 19880804
- 主分类号: A01K67/027
- IPC分类号: A01K67/027 ; C12N5/0735
摘要:
The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES) cells in culture. The ES cells are maintained and/or derived from animal embryos by culturing said cells or embryos in a culture medium containing an effective amount of LIF for a time and under conditions sufficient to maintain and/or derive said ES cells. The ES cells may be passaged in LIF and used to make chimaeric animals.
公开/授权文献
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