发明授权
- 专利标题: Plant promoter .alpha.-glucuronidase gene construct
- 专利标题(中): 植物启动子β-葡糖苷酸酶基因构建体
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申请号: US447976申请日: 1989-12-08
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公开(公告)号: US5268463A公开(公告)日: 1993-12-07
- 发明人: Richard A. Jefferson
- 申请人: Richard A. Jefferson
- 专利权人: Jefferson Richard A
- 当前专利权人: Jefferson Richard A
- 优先权: GBX8626862 19861111
- 主分类号: C07K14/245
- IPC分类号: C07K14/245 ; C12N9/24 ; C12N15/56 ; C12N15/82 ; C12N15/85
摘要:
The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.