发明授权
- 专利标题： Method for sequencing double-stranded DNA
- 专利标题（中）： 双链DNA测序方法
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申请号： US855293申请日： 1992-03-23
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公开(公告)号： US5308751A公开(公告)日： 1994-05-03
- 发明人: Tihiro Ohkawa , Robert L. Miller
- 申请人： Tihiro Ohkawa , Robert L. Miller
- 申请人地址： CA San Diego
- 专利权人： General Atomics
- 当前专利权人： General Atomics
- 当前专利权人地址： CA San Diego
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/50
摘要：
A method for simultaneously sequencing both strands of a target DNA is provided. The method involves "shifting" one or both strands of the target DNA by addition or deletion of one or more nucleotides to one or both strands of the duplex to produce shifted DNA. Both strands of the shifted DNA duplex and the target DNA are sequenced and the sequences of the shifted and unshifted target DNA are compared. Since portions of the sequenced strands of the target and shifted DNA are identical, all or a portion of sequence of the target DNA can be determined.
公开/授权文献
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