Invention Grant
- Patent Title: LTD.sub.4 receptor and photoaffinity probe
- Patent Title (中): LTD4受体和光亲和探针
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Application No.: US862692Application Date: 1992-04-03
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Publication No.: US5310884APublication Date: 1994-05-10
- Inventor: Robert Zamboni , Kathleen M. Metters
- Applicant: Robert Zamboni , Kathleen M. Metters
- Applicant Address: CAX Kirkland
- Assignee: Merck Frosst Canada, Inc.
- Current Assignee: Merck Frosst Canada, Inc.
- Current Assignee Address: CAX Kirkland
- Main IPC: C07K5/06
- IPC: C07K5/06 ; C07K5/072 ; C07K3/06 ; C07K7/00 ; C07K15/14
Abstract:
The LTD.sub.4 receptor has been defined by radioligand binding studies as a member of the family of G-protein coupled receptors. A photoactivable azido derivative of LTD.sub.4 ([.sup.125 I]Azido-LTD.sub.4) has been synthesized for use as a photoaffinity probe. Photoactivation of ([.sup.125 I]Azido-LTD.sub.4 under equilibrium binding conditions revealed the selective radiolabeling of a 45 kDa protein in guinea-pig lung membranes, as visualized by SDS-PAGE and autoradiography.
Public/Granted literature
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