发明授权
- 专利标题: Use of capillary electrophoresis for quantitating the concentration of protein components and of the total protein in fluids
- 专利标题(中): 使用毛细管电泳来定量流体中蛋白质组分和总蛋白质的浓度
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申请号: US489252申请日: 1995-06-14
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公开(公告)号: US5490909A公开(公告)日: 1996-02-13
- 发明人: Hann-Ping Wang , Cheng-Ming Liu
- 申请人: Hann-Ping Wang , Cheng-Ming Liu
- 申请人地址: CA Fullerton
- 专利权人: Beckman Instruments, Inc.
- 当前专利权人: Beckman Instruments, Inc.
- 当前专利权人地址: CA Fullerton
- 主分类号: G01N33/68
- IPC分类号: G01N33/68 ; G01N21/33 ; G01N21/79 ; G01N27/447 ; B01D57/00
摘要:
A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.