A machine for cleaning and disinfecting transportation vehicles comprises a frame provided with a plurality of wheels, electric motor means for driving the wheels and therefore moving the machine in a predetermined direction, a device for washing and cleaning a central area of the floor and under side seats of a transportation vehicle to be cleaned, and a device for washing and cleaning walls, seats and a ceiling of the transportation vehicle. The device for washing and cleaning the floor includes a unit for washing and cleaning a central area of the floor and a unit for washing and cleaning areas under the seats of the transportation vehicle, the device for washing and cleaning walls, seats, and ceiling being provided with lever elements movable between a plurality of positions suitable for correspondingly cleaning the walls, the seats and the ceiling of the transportation vehicle.