发明授权
US5534407A Method for nucleic acid hybridization using single-stranded DNA binding
protein
失效
使用单链DNA结合蛋白进行核酸杂交的方法
- 专利标题: Method for nucleic acid hybridization using single-stranded DNA binding protein
- 专利标题(中): 使用单链DNA结合蛋白进行核酸杂交的方法
-
申请号: US229396申请日: 1994-04-18
-
公开(公告)号: US5534407A公开(公告)日: 1996-07-09
- 发明人: Stanley Tabor , Charles C. Richardson
- 申请人: Stanley Tabor , Charles C. Richardson
- 申请人地址: MA Cambridge
- 专利权人: President and Fellows of Harvard College
- 当前专利权人: President and Fellows of Harvard College
- 当前专利权人地址: MA Cambridge
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12Q1/70
摘要:
Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.