Disclosed is a tamper-evident tape for applying over the flap or other opening in cellulosic substrates such as paper products, corrugated containers, cardboard boxes, shipping cartons and the like, which tape will provide evidence of tampering with its contents over a wide temperature range from above to below the Tg of the adhesive layer adhering the tape to the substrate. The tape has a transparent backing layer carrying on one side thereof a discontinuous layer of deposits of a transparent barrier material which is bonded to the backing layer and which prevents bonding of an underlying colored layer to the backing layer in areas where the barrier material is present.