发明授权
- 专利标题: Method and kit for making CDNA library
- 专利标题(中): 制作CDNA库的方法和试剂盒
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申请号: US406089申请日: 1995-03-17
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公开(公告)号: US5629179A公开(公告)日: 1997-05-13
- 发明人: Robert C. Mierendorf , Barbara B. Morris
- 申请人: Robert C. Mierendorf , Barbara B. Morris
- 申请人地址: WI Madison
- 专利权人: Novagen, Inc.
- 当前专利权人: Novagen, Inc.
- 当前专利权人地址: WI Madison
- 主分类号: C12N15/10
- IPC分类号: C12N15/10 ; C12Q1/68 ; C12P19/34 ; C07H21/04
摘要:
A method for forming directionally clonable randomly primed cDNA molecules includes the step of priming first strand synthesis using a set of first strand cDNA primers having the sequence 5'-XXNNNNNN-3' where XX is a constant dinucleotide pair across the set and NNNNNN is a random hexanucleotide, the set including primers representing all random hexanucleotides.
公开/授权文献
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