发明授权
- 专利标题: Process for preparing phosphorothioate oligonucleotides
- 专利标题(中): 制备硫代磷酸酯寡核苷酸的方法
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申请号: US993115申请日: 1992-12-18
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公开(公告)号: US5644048A公开(公告)日: 1997-07-01
- 发明人: Eric K. Yau
- 申请人: Eric K. Yau
- 申请人地址: CA Carlsbad
- 专利权人: ISIS Pharmaceuticals, Inc.
- 当前专利权人: ISIS Pharmaceuticals, Inc.
- 当前专利权人地址: CA Carlsbad
- 主分类号: C07H19/04
- IPC分类号: C07H19/04 ; C07H21/00
摘要:
A process for preparing phosphorothioate oligonucleotides utilizes S-(alkaryl or aryl) phosphorothioate compounds as intermediates suitable for use in the solution phase. A dinucleotide is prepared from a nucleoside intermediate that has been phosphitylated with an S-(alkaryl or aryl) alkyl phosphorothioate diester salt. The alkyl ester group is removed and the resulting nucleotide diester is reacted with a further nucleoside having a free 5'-hydroxyl group. Nucleotide units are linked together to yield compounds of the structure: ##STR1## where X is H, a first blocking group, a nucleoside, a nucleotide or an oligonucleotide; Y is H, a second blocking group, a nucleoside, a nucleotide or an oligonucleotide; R is an alkaryl or aryl group; n is an integer greater than 0; and Bx is a heterocyclic base. Deblocking of the S-alkaryl or S-aryl moiety yields a phosphorothioate oligonucleotide.