An isolator and method of using same for providing an atmosphere free of contamination from external sources and for containing and evacuating contaminates generated during the course of a surgical procedure, particularly laser procedures that are performed within the isolator. The isolator includes a double chambered structure made of a flexible material on a rigid frame. Filtered air is provided to the outer chamber and enters the inner chamber through various openings in the inner enclosure walls. The inner chamber is under negative pressure and the mix of filtered air and any air borne contaminants generated by the procedure are evacuated to an air handling unit where contaminants are neutralized and removed from the air. A portion of the top side of the bag is made of a relatively stiff, optically transparent, material through which the surgeon or the assembly worker can view the work area. A plurality of open-ended sleeves are attached to the bag through which the hands are arms of the surgeon and his assistants or the worker can extend into the work space.