发明授权
- 专利标题: Catalyzed reporter deposition
- 专利标题(中): 催化记者沉积
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申请号: US651012申请日: 1996-05-20
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公开(公告)号: US5731158A公开(公告)日: 1998-03-24
- 发明人: Mark Norman Bobrow , Gerald Joseph Litt
- 申请人: Mark Norman Bobrow , Gerald Joseph Litt
- 申请人地址: DE Wilmington
- 专利权人: E. I. du Pont de Nemours and Company
- 当前专利权人: E. I. du Pont de Nemours and Company
- 当前专利权人地址: DE Wilmington
- 主分类号: G01N33/532
- IPC分类号: G01N33/532 ; G01N33/535 ; G01N33/542 ; G01N33/58 ; G01N33/53
摘要:
The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.
公开/授权文献
- US5100809A Method of manufacturing semiconductor device 公开/授权日:1992-03-31