发明授权
US5744345A Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and
process for production
失效
超热稳定性β-半乳糖苷酶基因，由此编码的酶，以及生产方法
- 专利标题： Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and process for production
- 专利标题（中）： 超热稳定性β-半乳糖苷酶基因，由此编码的酶，以及生产方法
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申请号： US489733申请日： 1995-06-14
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公开(公告)号： US5744345A公开(公告)日： 1998-04-28
- 发明人: Atsushi Shimada , Miki Odate , Nobuto Koyama , Kimikazu Hashino , Kiyozo Asada , Ikunoshin Kato
- 申请人： Atsushi Shimada , Miki Odate , Nobuto Koyama , Kimikazu Hashino , Kiyozo Asada , Ikunoshin Kato
- 申请人地址： JPX Kyoto-fu
- 专利权人： Takara Shuzo Co., Ltd.
- 当前专利权人： Takara Shuzo Co., Ltd.
- 当前专利权人地址： JPX Kyoto-fu
- 优先权： JPX4-288156 19921005; JPX5-16846 19930108; JPX5-339188 19931206; JPX6-154356 19940614
- 主分类号： C12N9/38
- IPC分类号： C12N9/38 ; C12N15/56
摘要：
An isolated hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus is described. Examples include DNA sequences shown in SEQ ID NO. 2 and SEQ ID NO. 4 and DNAs hybridizable with these DNAs. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above sequences or portions thereof is used as a probe or primer is described. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above genes has been introduced is described. A particularly preferred gene encodes an SDS-resistant hyperthermostable .beta.-galactosidase.
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