A three-dimensional ROM device includes a silicon substrate having plurality of parallel trenches formed in an upper surface thereof, and a plurality of raised mesa regions. Each trench has a bottom and a pair of sidewalls, and is separated from an adjacent trench by a respective mesa region. A plurality of separated, parallel source/drain regions are provided, including a first and second source/drain region located on respective opposite sides of a respective trench bottom, and a third and fourth source/drain region located on respective opposite sides of a respective raised mesa region. Each source/drain region serves as a bit line. A gate oxide layer is located on the upper surface of the silicon substrate. A plurality of sidewall oxide layers are formed on selected sidewalls and serve as channel barriers. A plurality of silicon nitride layers are formed above selected mesa regions and trench bottoms, and serve as channel barriers. A plurality of gate layers are located over the gate oxide layer and the silicon nitride layers and serve as word lines. A region between any two adjacent source/drain regions comprises a channel region.