发明授权
- 专利标题: Human lymphotoxin
- 专利标题(中): 人淋巴毒素
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申请号: US243168申请日: 1994-05-16
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公开(公告)号: US5776446A公开(公告)日: 1998-07-07
- 发明人: Toshiaki Osawa , Masuo Obinata , Yoshiyuki Ishii , Yoshio Kobayashi
- 申请人: Toshiaki Osawa , Masuo Obinata , Yoshiyuki Ishii , Yoshio Kobayashi
- 申请人地址: JPX Tokyo
- 专利权人: Denki Kagaku Kogyo Kabushiki Kaisha
- 当前专利权人: Denki Kagaku Kogyo Kabushiki Kaisha
- 当前专利权人地址: JPX Tokyo
- 优先权: JPX60-289249 19851224; JPX61-151772 19860630; JPX61-151773 19860630; JPX62-160115 19870627
- 主分类号: A61K38/00
- IPC分类号: A61K38/00 ; C07K14/525 ; A61K38/19 ; C07K14/52
摘要:
Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.
公开/授权文献
- US4954483A Carboxylic acid ester derivatives or deglucoteicoplanin 公开/授权日:1990-09-04