发明授权
- 专利标题: Commercial production of .beta.-glucuronidase in plants
- 专利标题(中): 在植物中商业生产β-葡糖苷酸酶
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申请号: US554169申请日: 1995-11-06
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公开(公告)号: US5804694A公开(公告)日: 1998-09-08
- 发明人: Wesley B. Bruce , Elizabeth Hood , David J. Peterson , James C. Register, III , Derrick Witcher , John A. Howard
- 申请人: Wesley B. Bruce , Elizabeth Hood , David J. Peterson , James C. Register, III , Derrick Witcher , John A. Howard
- 申请人地址: TX College Station
- 专利权人: ProdiGene, Inc.
- 当前专利权人: ProdiGene, Inc.
- 当前专利权人地址: TX College Station
- 主分类号: C12N9/24
- IPC分类号: C12N9/24 ; C12N15/55 ; C12N15/82 ; C12N15/29 ; A01H4/00 ; A01H5/00
摘要:
A method for commercial production of GUS entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the GUS-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of GUS expression and that can be used as a site of integration for high level expression of other genes of interest.
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