发明授权
- 专利标题: Automated DNA sequencing technique
- 专利标题(中): 自动DNA测序技术
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申请号: US361176申请日: 1994-12-21
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公开(公告)号: US5821058A公开(公告)日: 1998-10-13
- 发明人: Lloyd M. Smith , Leroy E. Hood , Michael W. Hunkapiller , Tim J. Hunkapiller , Charles R. Connell
- 申请人: Lloyd M. Smith , Leroy E. Hood , Michael W. Hunkapiller , Tim J. Hunkapiller , Charles R. Connell
- 申请人地址: CA Pasadena
- 专利权人: California Institute of Technology
- 当前专利权人: California Institute of Technology
- 当前专利权人地址: CA Pasadena
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N27/447 ; C07H21/04 ; C12P19/34
摘要:
A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. Preferably four different fragment sets are tagged with the fluorophores fluorescein, Texas Red, tetramethyl rhodamine, and 7-nitrobenzofurazan. A system for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations comprising: a source of chromophore or fluorescent tagged DNA fragments; a zone for contacting an electrophoresis gel; means for introducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.
公开/授权文献
- US5747249A Automated DNA sequencing technique 公开/授权日:1998-05-05