发明授权
US5821060A DNA sequencing, mapping, and diagnostic processes using hybridization
chips and unlabeled DNA
失效
使用杂交芯片和未标记的DNA进行DNA测序,绘图和诊断过程
- 专利标题: DNA sequencing, mapping, and diagnostic processes using hybridization chips and unlabeled DNA
- 专利标题(中): 使用杂交芯片和未标记的DNA进行DNA测序,绘图和诊断过程
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申请号: US691614申请日: 1996-08-02
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公开(公告)号: US5821060A公开(公告)日: 1998-10-13
- 发明人: Heinrich F. Arlinghaus , K. Bruce Jacobson
- 申请人: Heinrich F. Arlinghaus , K. Bruce Jacobson
- 申请人地址: TN Oak Ridge
- 专利权人: Atom Sciences, Inc.
- 当前专利权人: Atom Sciences, Inc.
- 当前专利权人地址: TN Oak Ridge
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C40B60/14 ; B01D59/44 ; G01N33/48
摘要:
A process for deoxyribonucleic acid (DNA) sequencing, mapping, and diagnostics which utilizes the differences between the chemical composition of DNA and that of peptide nucleic acids (PNAs) to provide DNA sequencing, mapping, or diagnostics using natural DNA fragments, rather than using radioisotopes, stable isotopes or fluorescent substances to label the DNAs. The process includes the steps of hybridizing PNA segments to complementary DNA segments which are fixed to a hybridization surface, or hybridizing DNA segments to complementary PNA segments which are fixed to a hybridization surface, and using mass spectrometric or non-mass spectrometric techniques to analyze the extent of hybridization at each potential hybridization site.