发明授权
- 专利标题： Cloning vector, polylinker and methods
- 专利标题（中）： 克隆载体，多接头和方法
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申请号： US861205申请日： 1997-05-21
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公开(公告)号： US5830731A公开(公告)日： 1998-11-03
- 发明人: Brian Seed , Alejandro Aruffo
- 申请人： Brian Seed , Alejandro Aruffo
- 申请人地址： MA Charlestown
- 专利权人： The General Hospital Corporation
- 当前专利权人： The General Hospital Corporation
- 当前专利权人地址： MA Charlestown
- 主分类号： A61K38/00
- IPC分类号： A61K38/00 ; C07K14/705 ; C12N15/10 ; C12N15/12 ; C12N15/85 ; G01N33/569 ; C12N15/66
摘要：
A simple and highly efficient method for cloning cDNAs from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.
公开/授权文献
- US5267326A Bitmap image segmentation using a charge model for pixels 公开/授权日：1993-11-30
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