发明授权
- 专利标题： Differential inactivation of nucleic acids by chemical modification
- 专利标题（中）： 化学修饰对核酸的差异失活
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申请号： US587725申请日： 1996-01-19
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公开(公告)号： US5843459A公开(公告)日： 1998-12-01
- 发明人: Suming Wang , Charles J. Link, Jr.
- 申请人： Suming Wang , Charles J. Link, Jr.
- 申请人地址： IA Des Moines
- 专利权人： Human Gene Therapy Research Institute
- 当前专利权人： Human Gene Therapy Research Institute
- 当前专利权人地址： IA Des Moines
- 主分类号： C12N15/869
- IPC分类号： C12N15/869 ; C12Q1/70 ; A61K39/245
摘要：
Disclosed herein is the method for dramatically reducing cytotoxicity of viral vectors such as Herpes simplex viral while retaining gene expression. The method of the invention virtually eliminates the concern of possible recombination during virus propagation and contamination of wild-type virus and virus stock. The invention comprises use of photochemical crosslinking causing differential inactivation of viruses.