发明授权
- 专利标题: Isolated native primate GM-CSF protein
- 专利标题(中): 分离的天然灵长类动物GM-CSF蛋白
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申请号: US344809申请日: 1994-11-23
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公开(公告)号: US5895646A公开(公告)日: 1999-04-20
- 发明人: Elizabeth A. Wang
- 申请人: Elizabeth A. Wang
- 申请人地址: CHX Basel
- 专利权人: Novartis Corporation
- 当前专利权人: Novartis Corporation
- 当前专利权人地址: CHX Basel
- 主分类号: A61K38/00
- IPC分类号: A61K38/00 ; C07K14/535 ; C12N15/70 ; C12N15/81 ; C12N15/85 ; C07K1/26
摘要:
A method for purifying CSF protein is described. The method comprises: precipitating the protein with ammonium sulfate at 80% saturation to form a pellet containing the CSF protein; resuspending the pellet in a buffered solution at a pH in the range of about 6 to about 8; applying the buffered solution containing CSF to a chromatographic column, eluting with the buffered solution containing sodium chloride and collecting the fractions having CSF activity; pooling the active fractions, applying them to a C4 reverse phase column and eluting with a 0 to 90% acetonitrile gradient to collect the active fractions. The purified CSF protein has a specific activity of at least about 1.times.10.sup.7 units per mg of protein and preferably at least about 4.times.10.sup.7 units per mg of protein when assayed using the human bone marrow assay.