发明授权
US5916777A Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
失效
使用3'-核糖核苷酸引物进行寡核苷酸的酶合成
- 专利标题： Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
- 专利标题（中）： 使用3'-核糖核苷酸引物进行寡核苷酸的酶合成
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申请号： US949076申请日： 1997-10-10
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公开(公告)号： US5916777A公开(公告)日： 1999-06-29
- 发明人: Daniel Louis Kacian , Nanibhushan Dattagupta , David Bruce Lackey
- 申请人： Daniel Louis Kacian , Nanibhushan Dattagupta , David Bruce Lackey
- 申请人地址： CA San Diego
- 专利权人： Gen-Probe Incorporated
- 当前专利权人： Gen-Probe Incorporated
- 当前专利权人地址： CA San Diego
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12Q1/68 ; A01N43/04 ; C07H21/04 ; C12P19/34
摘要：
The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.
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