发明授权
- 专利标题: Assay devices comprising a porous capture membrane in fluid-withdrawing contact with a nonabsorbent capillary network
- 专利标题(中): 测定装置包括与非吸收性毛细管网流体吸收接触的多孔捕获膜
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申请号: US458276申请日: 1995-06-02
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公开(公告)号: US5922615A公开(公告)日: 1999-07-13
- 发明人: Mark R. Nowakowski , Kenneth F. Buechler , Richard R. Anderson , Gunars E. Valkirs
- 申请人: Mark R. Nowakowski , Kenneth F. Buechler , Richard R. Anderson , Gunars E. Valkirs
- 申请人地址: CA San Diego
- 专利权人: Biosite Diagnostics Incorporated
- 当前专利权人: Biosite Diagnostics Incorporated
- 当前专利权人地址: CA San Diego
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; G01N33/543 ; G01N33/558 ; G01N37/00
摘要:
Devices for use in heterogeneous ligand-receptor assays, having a porous member in contact with a nonabsorbent textured surface, where the surface texturing is such that a capillary network is formed when in fluid communication with the porous member. More particularly, these devices comprise:(a) a porous member having (i) at least one binding agent capable of immobilizing at least one target ligand on the porous member from a fluid sample in at least one zone and (ii) a means for detecting the presence or amount of said target ligand as a result of the assay process; and(b) a nonabsorbent member in fluid communication with the porous member, the nonabsorbent member forming at least one capillary with the porous member so that when sample, alone or in combination with other fluids, is added to the porous member, fluid is drawn through the porous member.
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