发明授权
US5962326A Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and
process for production
失效
超热稳定性β-半乳糖苷酶基因，由此编码的酶，以及生产方法
- 专利标题： Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and process for production
- 专利标题（中）： 超热稳定性β-半乳糖苷酶基因，由此编码的酶，以及生产方法
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申请号： US993581申请日： 1997-12-18
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公开(公告)号： US5962326A公开(公告)日： 1999-10-05
- 发明人: Atsushi Shimada , Miki Odate , Nobuto Koyama , Kimikazu Hashino , Kiyozo Asada , Ikunoshin Kato
- 申请人： Atsushi Shimada , Miki Odate , Nobuto Koyama , Kimikazu Hashino , Kiyozo Asada , Ikunoshin Kato
- 专利权人： Shimada; Atsushi,Odate; Miki,Koyama; Nobuto,Hashino; Kimikazu,Asada; Kiyozo,Kato; Ikunoshin
- 当前专利权人： Shimada; Atsushi,Odate; Miki,Koyama; Nobuto,Hashino; Kimikazu,Asada; Kiyozo,Kato; Ikunoshin
- 优先权： JPX4-288156 19921005; JPX5-16846 19930108; JPX5-339188 19931206; JPX6-154356 19940614
- 主分类号： C12N9/38
- IPC分类号： C12N9/38 ; C12N15/56 ; C12N15/10 ; C12N15/63
摘要：
An isolated SDS-resistant hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus. Examples thereof include genes respectively shown in SEQ ID NO: 1 and SEQ ID NO: 2 and genes each hybridizable with the above gene shown in SEQ ID NO: 1. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above genes or pacts thereof is used as a probe or primer. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above gene has been introduced.
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