A patient is first injected with a contrast agent. A complete scan along a plurality of scan lines is then performed, resulting in the destruction of some contrast agent. While some of the contrast agent is destroyed, non-scanned portions of the target, and possibly the scan region, still include at least some contrast agent. A second scan is then sequentially performed sufficiently close in time to the first scan such that reflections from the anatomy remain unchanged. The second frame of data from the second scan will differ from the first frame of data from the first scan by only contrast agent effects. The second frame of data is subtracted from the first frame of data. The resulting data is used to image the contrast agent. By varying the time between each pair of scans, differences in the speed of the contrast agent motion within the body may be assessed. The collateral damage of contrast agents from ultrasound acoustic energy is minimized while the degradation due to various changes is also minimized.