发明授权
- 专利标题： Process for preparing hepatitis C virus envelope glycoproteins
- 专利标题（中）： 制备丙型肝炎病毒包膜糖蛋白的方法
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申请号： US334545申请日： 1994-11-04
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公开(公告)号： US5985609A公开(公告)日： 1999-11-16
- 发明人: Mi-Kyung Min , Joon-Sang Park , Jung-Seob Kim , Yung-Dae Yun , Hong-Mo Moon
- 申请人： Mi-Kyung Min , Joon-Sang Park , Jung-Seob Kim , Yung-Dae Yun , Hong-Mo Moon
- 申请人地址： KRX Kyonggi-Do
- 专利权人： Mogam Biotechnology Research Institute
- 当前专利权人： Mogam Biotechnology Research Institute
- 当前专利权人地址： KRX Kyonggi-Do
- 主分类号： A61K39/00
- IPC分类号： A61K39/00 ; C07K14/18 ; C12N15/51 ; C07H21/04 ; C12Q1/70
摘要：
The present invention relates to a novel process for preparing hepatitis C virus (HCV) envelope glycoproteins employing Chinese Hamster Ovary (CHO) cells transformed with recombinant expression vectors containing the hepatitis C virus genome. The present invention provides CHO cells cotransfected with DHFR (dihydrofolate reductase) minigene pDCHIP and recombinant expression vectors containing cDNAs of HCV E1 and E2/NS1 ligated with tissue plasminogen activator signal sequence. HCV E1 and E2/NS1 envelope glycoproteins are produced in a massive manner from the transformed CHO cells adapted in methotrexate. The HCV envelope glycoproteins produced by the present invention can be applied to the development of a diagnostic reagent and a potential preventive HCV vaccine.
公开/授权文献
- US4466004A Thermomagnetic recording 公开/授权日：1984-08-14
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