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US5998213A C-terminal sequencing of peptides which may include proline 失效
肽的C末端测序可能包括脯氨酸

C-terminal sequencing of peptides which may include proline
摘要:
A carboxy terminal protein sequencing process is disclosed which utilizes diphenyl phosphoroisothiocyanatidate and a heterocyclic amine to produce a thiohydantoin derivative of the C-terminal amino acid. The derivative is readily cleaved. The method is useful to sequence through all of the 20 naturally occuring amino acids.
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