发明授权
- 专利标题： Silent inducible virus replicons and uses thereof
- 专利标题（中）： 沉默诱导型病毒复制子及其用途
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申请号： US227556申请日： 1999-01-08
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公开(公告)号： US6136538A公开(公告)日： 2000-10-24
- 发明人: Paul D. Olivo , Sondra Schlesinger
- 申请人： Paul D. Olivo , Sondra Schlesinger
- 申请人地址： MO St. Louis
- 专利权人： Washington University
- 当前专利权人： Washington University
- 当前专利权人地址： MO St. Louis
- 主分类号： C12N5/10
- IPC分类号： C12N5/10 ; C12N15/40 ; C12N15/85 ; C12N15/86 ; C12Q1/68 ; C12Q1/70 ; C07H21/04 ; A61K48/00
摘要：
A recombinant cell stably transformed with a cDNA of a silent, inducible replicon encoding a recombinant protein is disclosed. Transcription of the replicon cDNA is under the control of a silent promoter inducible by a DNA virus and expression of the recombinant protein is dependent upon the presence of the DNA virus in the cell. The cell can be engineered to package the replicon upon infection by the DNA virus, leading to intercellular amplification of expression of the recombinant protein. Where the recombinant protein is a reporter gene product, the recombinant cell may be used in an assay for detecting DNA viruses. A kit for performing such an assay is also provided.
公开/授权文献
- US4385857A Intermodal transport system 公开/授权日：1983-05-31
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