发明授权
- 专利标题: Expression of G protein coupled receptors in yeast
- 专利标题(中): 酵母中G蛋白偶联受体的表达
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申请号: US09056920申请日: 1998-04-08
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公开(公告)号: US06168927A公开(公告)日: 2001-01-02
- 发明人: Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
- 申请人: Klim King , Henrik G. Dohlman , Marc G. Caron , Robert J. Lefkowitz
- 主分类号: G01N3353
- IPC分类号: G01N3353
摘要:
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein &agr; subunit (mammalian G&agr;). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein &agr;-subunit (yeast G&agr;) The cells are useful for screening compounds which affect the rate of dissociation of G&agr; from G&bgr;&ggr; in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor.