A printing system having a registration system for use with a variety of different types of media. The printing system is suitable for use with labels (with gaps or I-marks), continuous plastic (with I-marks), and bags (with perforations). The printing system includes a system for registering perforations (30) including a vacuum probe (34), vacuum sensor (36) and detection circuitry (38) to detect perforations. The printing system also includes a system for registering contrasting I-marks (10) appearing on a transparent media.