发明授权
- 专利标题: Photon reducing agents for use in fluorescence assays
- 专利标题(中): 用于荧光测定的光子还原剂
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申请号: US09122477申请日: 1998-07-23
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公开(公告)号: US06221612B1公开(公告)日: 2001-04-24
- 发明人: Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
- 申请人: Tom Knapp , Gregor Zlokarnik , Paul Negulescu , Roger Y. Tsien , Tim Rink
- 主分类号: C12Q170
- IPC分类号: C12Q170
摘要:
The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.