发明授权
US06232094B1 DNA encoding human &kgr; casein and process for obtaining the protein
失效
编码人类kappa酪蛋白的DNA和获得蛋白质的方法
- 专利标题: DNA encoding human &kgr; casein and process for obtaining the protein
- 专利标题(中): 编码人类kappa酪蛋白的DNA和获得蛋白质的方法
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申请号: US08462437申请日: 1995-06-05
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公开(公告)号: US06232094B1公开(公告)日: 2001-05-15
- 发明人: Lennart Hansson , Mats Strömqvist , Sven Bergström , Olle Hernell , Jan Törnell
- 申请人: Lennart Hansson , Mats Strömqvist , Sven Bergström , Olle Hernell , Jan Törnell
- 优先权: DK88/92 19920123
- 主分类号: C12N2106
- IPC分类号: C12N2106
摘要:
The present invention relates to an expression system comprising a DNA sequence encoding a polypeptide which has a biological activity of human &kgr;-casein, the system comprising a 5′-flanking sequence capable of mediating expression of said DNA sequence. In preferred embodiments the 5′-flanking sequence is from a milk protein gene of a mammal such as a casein gene or whey acidic protein (WAP) gene and the DNA sequence contains at least one intron sequence selected from the intron sequences presented in SEQ ID NO:30. The invention also relates to DNA sequences, replicable expression vectors and cells harboring said vectors, recombinant polypeptide e.g. in glycosylated form, and milk, infant formula or nutrient supplement comprising recombinant polypeptide. The invention further relates to a transgenic non-human mammal such as a mouse, rat, rabbit, goat, sheep, pig, lama, camel or bovine species whose germ cells and somatic cells contain a DNA sequence as defined above as a result of chromosomal incorporation into the non-human mammalian genome, or into the genome of an ancestor of said non-human mammal.