发明授权
US06258556B1 cDNA and genomic clones encoding human &mgr; opiate receptor and the purified gene product
失效
cDNA和基因组克隆编码人类鸦片剂受体和纯化的基因产物
- 专利标题： cDNA and genomic clones encoding human &mgr; opiate receptor and the purified gene product
- 专利标题（中）： cDNA和基因组克隆编码人类鸦片剂受体和纯化的基因产物
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申请号： US08188275申请日： 1994-01-28
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公开(公告)号： US06258556B1公开(公告)日： 2001-07-10
- 发明人: George Uhl , Peter Johnson , Antonio M. Persico , Jia Bei Wang
- 申请人： George Uhl , Peter Johnson , Antonio M. Persico , Jia Bei Wang
- 主分类号： C12P2106
- IPC分类号： C12P2106
摘要：
A human &mgr; opiate receptor cDNA has been identified from a cerebral cortical CDNA library using sequences from the rat &mgr; opiate receptor CDNA. The human &mgr; opiate receptor (h&mgr;OR1) shares 95% amino acid identity with the rat sequence. The expressed &mgr;OR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat &mgr; opiate receptor. Effects on cyclic AMP are similar to those noted for the rat &mgr; opiate receptor. Overlapping genomic clones spanning 50 kilobasepairs and hybridizing with the h&mgr;OR1 cDNA contains exon sequences encoding the entire open reading frame of the human A opiate receptor are described. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, is being used to assess this gene's involvement in neuropsychiatric disorders involving opiatergic systems.
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