发明授权
US06291181B1 Nucleic acid adapters containing a type IIs restriction site and methods of using the same
有权
含有II型限制性位点的核酸衔接子及其使用方法
- 专利标题： Nucleic acid adapters containing a type IIs restriction site and methods of using the same
- 专利标题（中）： 含有II型限制性位点的核酸衔接子及其使用方法
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申请号： US09412246申请日： 1999-10-05
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公开(公告)号： US06291181B1公开(公告)日： 2001-09-18
- 发明人: Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
- 申请人： Ronald J. Sapolsky , Robert J. Lipshutz , Thomas R. Gingeras
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
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