发明授权
US06291191B1 Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization
有权
用于配体开发和多变量蛋白质化学优化的微板热转移测定
- 专利标题： Microplate thermal shift assay for ligand development and multi-variable protein chemistry optimization
- 专利标题（中）： 用于配体开发和多变量蛋白质化学优化的微板热转移测定
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申请号： US09452203申请日： 1999-12-02
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公开(公告)号： US06291191B1公开(公告)日： 2001-09-18
- 发明人: Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
- 申请人： Michael W. Pantoliano , Eugenio C. Petrella , Alexander W. Rhind , Francis R. Salemme , Barry A. Springer
- 主分类号： G01N3353
- IPC分类号： G01N3353
摘要：
The present invention is a method for ranking the affinity of each of a multiplicity of different molecules for a target molecule which is capable of denaturing due to a thermal change. The method comprises contacting the target molecule with one molecule of the multiplicity of different molecules in each of a multiplicity of containers, simultaneously heating the multiplicity of containers, measuring in each of the containers a physical change associated with the thermal denaturation of the target molecule resulting from the heating in each of the containers, generating a thermal denaturation curve for the target molecule as a function of temperature for each of the containers and determining a midpoint temperature (Tm) therefrom, comparing the Tm of each of the thermal denaturation curves with the Tm of a thermal denaturation curve obtained for the target molecule in the absence of any of the molecules in the multiplicity of different molecules, and ranking the affinities of the multiplicity of different molecules according the change in Tm of each of the thermal denaturation curves.
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