发明授权
US06300543B1 Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
有权
受精卵,卵或精子细胞的转化以及从分离的胚囊恢复转化的植物
- 专利标题: Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
- 专利标题(中): 受精卵,卵或精子细胞的转化以及从分离的胚囊恢复转化的植物
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申请号: US09147489申请日: 1999-05-12
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公开(公告)号: US06300543B1公开(公告)日: 2001-10-09
- 发明人: David D. Cass , Locksley E. McGann , Guichang Zhang , John D. Laurie , Jerome P. Ranch , William J. Gordon-Kamm
- 申请人: David D. Cass , Locksley E. McGann , Guichang Zhang , John D. Laurie , Jerome P. Ranch , William J. Gordon-Kamm
- 主分类号: C12N1582
- IPC分类号: C12N1582
摘要:
Methodology is provided for the production of uniformly transformed plants capable of transmitting a foreign gene to progeny by sexual reproduction. A foreign gene is introduced into the zygote in an isolated embryo sac and a transformed plant is recovered. Alternatively, a foreign gene is introduced into an egg cell in an isolated embryo sac, the egg cell is fertilized with an isolated sperm cell and a transformed plant is recovered. Sperm cells may be transformed with a foreign gene, an egg cell in an isolated embryo sac is fertilized with the transformed sperm cells, or nuclei isolated from the transformed sperm cells, and a transgenic plant is recovered. Another method for the production of transgenic plants is transformation of an embryo in an isolated embryo sac. The transgenic plant produced by any one of these methods is homogeneously transformed and capable of transmitting the foreign gene to progeny by sexual reproduction.
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