发明授权
- 专利标题： Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
- 专利标题（中）： 使用不对称结构在生物电子微芯片上多重扩增和分离核酸序列
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申请号： US09290452申请日： 1999-04-12
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公开(公告)号： US06309833B1公开(公告)日： 2001-10-30
- 发明人: Carl F. Edman , Michael I. Nerenberg , Lorelei P. Westin , John J. Carrino
- 申请人： Carl F. Edman , Michael I. Nerenberg , Lorelei P. Westin , John J. Carrino
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.
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