发明授权
US06337190B1 D-amino acid aminotransferase for simultaneously producing glutaryl-7-aminocephalosporanic acid and D-amino acid
失效
同时生产戊二酰-7-氨基头孢烷酸和D-氨基酸的D-氨基酸氨基转移酶
- 专利标题： D-amino acid aminotransferase for simultaneously producing glutaryl-7-aminocephalosporanic acid and D-amino acid
- 专利标题（中）： 同时生产戊二酰-7-氨基头孢烷酸和D-氨基酸的D-氨基酸氨基转移酶
-
申请号： US09466257申请日： 1999-12-17
-
公开(公告)号： US06337190B1公开(公告)日： 2002-01-08
- 发明人: Tzann-Shun Hwang , Szu-Pei Wu , Hsin-Hua Chou , Hwa-Yi Chen , Lung-Shen Lin , Hsin Tsai , Edward Chang
- 申请人： Tzann-Shun Hwang , Szu-Pei Wu , Hsin-Hua Chou , Hwa-Yi Chen , Lung-Shen Lin , Hsin Tsai , Edward Chang
- 主分类号： C12N900
- IPC分类号： C12N900
摘要：
The present invention relates to mutant D-amino acid aminotransferase, including nucleic acids encoding mutant D-amino acid aminotransferase. The mutant D-amino acid aminotransferase of the present invention is obtained by the substitution of the glutamate residue at position 13 of wild type D-amino acid aminotransferase from Bacillus sphaericus with hydrophobic amino acids. The mutant D-amino acid aminotransferase can be used in the production of D-amino acid and the conversion of glutaryl-7-aminocephalosporanic acid from cephalosporin C. The present invention also includes replica-paper staining method for screening the cells expressing high DAT activity.
信息查询
