发明授权
US06338964B1 Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
有权
在低溶解二氧化碳浓度下哺乳动物细胞培养的方法和培养基
- 专利标题： Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
- 专利标题（中）： 在低溶解二氧化碳浓度下哺乳动物细胞培养的方法和培养基
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申请号： US09307175申请日： 1999-05-07
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公开(公告)号： US06338964B1公开(公告)日： 2002-01-15
- 发明人: Ricaredo Matanguihan , Eva Sajan , Konstantin Konstantinov , Michael Zachariou , Charles Olson
- 申请人： Ricaredo Matanguihan , Eva Sajan , Konstantin Konstantinov , Michael Zachariou , Charles Olson
- 主分类号： C12N500
- IPC分类号： C12N500
摘要：
A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing agent. The medium is preferably essentially free of added sodium bicarbonate. Methods of use of the medium in culturing mammalian cells, particularly cells engineered to produce recombinant factor VIII, are also disclosed.
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