An on-chip system and method for calibrating an illumination source includes a photo-detector and intensity sense and control circuitry resident on an integrated circuit. The integrated circuit is illuminated by an illumination source, which impinges upon the photo-detector. The intensity sense and control circuitry receives the measured intensity value of the illumination source and compares the measured intensity to a predetermined value representing the desired intensity. Subject to a range of operation, the intensity sense and control circuitry adjusts the intensity of the illumination source based upon the difference between the measured illumination intensity and the desired illumination intensity.