发明授权
- 专利标题： Single primer PCR amplification of RNA
- 专利标题（中）： RNA的单引物PCR扩增
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申请号： US09617578申请日： 2000-07-17
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公开(公告)号： US06379932B1公开(公告)日： 2002-04-30
- 发明人: Lyle Arnold , Erik Bjeldanes , Steve Daniel
- 申请人： Lyle Arnold , Erik Bjeldanes , Steve Daniel
- 主分类号： C12P1934
- IPC分类号： C12P1934
摘要：
The invention provides methods and compositions for amplifying RNA sequences by (a) hybridizing to a target RNA a first primer comprising a 3′ target RNA hybridizing sequence and a first 5′ defined amplifyable sequence; (b) extending the first primer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the first cDNA strand a second primer comprising a 3′ random cDNA hybridizing sequence and a second 5′ defined amplifyable sequence; (d) extending the second primer with a DNA polymerase to form a second cDNA strand; and (e) amplifying the second cDNA strand with a third primer comprising the first 5′ defined amplifyable sequence.
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